Resources

Ilaria Angeloni et al.
Nanoscale

Mariana P. Brandao et al.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of the optical excitation from an excited electronic level of one molecule, called the donor, to a resonant electronic level of another molecule, called the acceptor. In this application note we show that one can increase the method sensitivity and reduce cost of the assay by applying a time-resolved fluorescence format to the FRET-based immunoassay.

Roger Fenske, Dirk U. Näther, Richard B. Dennis, S. Desmond Smith

Commercial Fluorescence Lifetime Spectrometers have long suffered from the lack of a simple, compact and relatively inexpensive broad spectral band light source that can be flexibly employed for both quasi-steady state and time resolved measurements (using Time Correlated Single Photon Counting [TCSPC]). This paper reports the integration of an optically pumped photonic crystal fibre, supercontinuum source1 (Fianium model SC400PP) as a light source in Fluorescence Lifetime Spectrometers (Edinburgh Instruments FLS920 and Lifespec II), with single photon counting detectors (micro-channel plate photomultiplier and a near-infrared photomultiplier) covering the UV to NIR range. An innovative method of spectral selection of the supercontinuum source involving wedge interference filters is also discussed.