DS5 Dual Beam UV-Vis Spectrophotometer
- Variable bandpass options at 0.5, 1.0, 1.5, 2.0 or 4.0 nm over
the range 190 nm – 1100 nm - Fast scanning – up to 6,000 nm/min to aid sample analysis throughput
- Automatic accessory recognition connected to the DS5
- Optional Visacle® software for PC control
- Modern touchscreen interface with intuitive menus and functions to ensure ease of use for standalone control
- USB, SD card and internal data storage for convenient retrieval of methods and results
Product Description
DS5 Dual Beam UV-Vis Spectrophotometer
The DS5 UV-Vis Spectrophotometer is a high performance dual beam instrument that measures absorption and transmission as a function of wavelength and is suitable for many analytical applications where accuracy and precision measurements are key to your results.
Designed and manufactured in the UK, the DS5 provides a modern, user-friendly and accurate UV-Vis spectrophotometer for a wide range of sample types and measurements.
Utilising a dual lamp and Czerny-Turner configuration monochromator, the DS5 features a compact, reliable and high throughput optical system which ensures impressive spectral performance. Additional benefits include stray light, baseline flatness, wavelength and photometric accuracy and reproducibility.
Application Note – “Transmission and Absorbance Measurements of Liquid Samples”
The DS5 can be used for absorbance measurements, an example of the type of research which can be carried out can be found in our application note “Transmission and Absorbance Measurements of Liquid Samples”. You can download the full application note here.
For price details, further information or to find out how our UV-Vis spectrophotometer can assist you with your research, simply contact a member of our sales team at sales@edinst.com.
Technical Specifications
| Parameter | Description |
|---|---|
| Optics | Czerny-Turner, Dual Beam Monochromator |
| Wavelength Range | 190 nm – 1100 nm |
| Spectral Bandwidth | 0.5 nm, 1 nm, 1.5 nm, 2 nm and 4 nm |
| Stray Light | ≤0.10% T (220 nm NaI, 340 nm NaNO2) |
| Wavelength Accuracy | ±0.1 nm |
| Wavelength Repeatability | ±0.1 nm |
| Photometric Range | Absorbance: -3.4 to +3.4, %T: 0 to 300, Concentration: 0,000 to 9,999 |
| Photometric Accuracy | ±0.002 Abs (0 – 0.5 Abs), ±0.004 Abs (0.5 – 1.0 Abs), ±0.008 Abs (1.0 – 2.0 Abs) |
| Photometric Reproducibility | ±0.0003 Abs (0-0.5 Abs), ±0.0005 Abs (0.5-1 Abs), ±0.001 Abs (1.0-2 Abs) |
| Wavelength Scan Speed | 10, 100, 200, 400, 800, 1200, 2400, 3600, 6000 nm/min |
| RMS Noise | ≤0.00003 Abs (0 Abs, 500nm) |
| Baseline Stability | <0.0003 Abs/hr (500 nm, 2 hour lamp warm-up period) |
| Baseline Flatness | ±0.0009 Abs (200 nm – 950 nm) |
| Light Source | Tungsten-Halogen and Deuterium Lamps |
| Light Source Switching | Automatic switching selectable for 325 nm – 370 nm range |
| Detector | Silicon Photodiode |
| Display | 7” Touchscreen |
| Software | Touchscreen operation, PC-controlled software or CFR compliant PC-controlled software |
| Dimensions | 500 mm (W) × 475 mm (D) × 250 mm (H) |
| Net Weight | 20 Kg (approx) |
| Power Supply | 100 – 240V, 50/60 Hz, 150VA |
| Ambient Temperature | 10°C – 35°C |
| Output Device | USB flash drive, SD card |
| Interface | USB PC interface |
Software
| Home Screen | Measurement Setup |
 |
 |
UV-Vis Measurement Modes
Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single up to 5 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves for concentration measurements.
Time Scan: Perform kinetic measurements for time periods ranging from 10 seconds to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.
Wavelength scan: Perform a full spectral scan from 190 nm – 1100 nm at any of 9 incremental and preset selectable scan speeds starting from a high resolution 10 nm/min up to a maximum scan speed of 6,000 nm/min. Data is displayed as a graphical spectrum on which data analysis can be performed through the touchscreen interface with zoom, peak-valley, smoothing and other functions available through the easy to use menu interface.
- Concentration measurement
- Absorbance/Transmittance measurement
- Hexavalent Chromium, Nucleic acid measurement (nucleic acid purity, nucleic acid concentration, protein concentration calculation)
- Wavelength scan
- Time change
- Single and multi-wavelength photometry
| Spectrum Overlay | Area Calculation |
 |
 |
Validation Functions
To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the DS5 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can all be validated.
Data Handling
- Scale changing, Trace, Spectrum overlay, Peak & trough detection, Smoothing, Differentiation, and Area & rate calculating
- Internal memory, External USB, SD card
- File output in CSV and text format
Optional Visacle® PC-controlled Software
Visacle® offers all control, validation, data analysis and reporting functionality for DS5 in one PC-operated software package. Collected data is stored in a secure database, where it can then be organised and analysed, as well as exported in .txt, .csv or PDF format.
Visacle® can be purchased as a CFR Part 11 compliant package for users requiring additional security. Features include:
- Configurable user profiles with secure login and e-signatures
- Audit trail logging of all actions taken
- Permission system to restrict actions and data access to authorised users
| Visacle® Project Browser | Visacle® CFR User Profile Manager |
 |
 |
