Steady State Fluorescence
Steady State Fluorescence Spectroscopy investigates the long-term average fluorescence of a sample when irradiated with UV, Visible or near-IR Light.
Edinburgh Instruments offers a range of research grade and analytical steady state spectrofluorometers. These vary in a number of ways but can be compact, benchtop or modular, and fully customisable for any type of fluorescence measurements that will meet the most demanding research requirements. For further information or to discuss your requirements, please contact us.
Description
The fluorescence spectrum is a plot of fluorescence intensity vs. the registered wavelength (energy and frequency) at one excitation wavelength. These fluorescence intensity measurements (spectral measurements) allow the determination of the presence of fluorophores and their concentrations.
Spectral measurements are made using a technique called Single Photon Counting (SPC). All instruments manufactured by Edinburgh Instruments detect photons by using suitable Photomultiplier Tubes (PMTs) detectors. Using PMTs and single photon counting allows for the ultimate in accuracy and sensitivity in the acquisition of data.
Steady State measurement examples include:
- Excitation and Emission Scans
- Synchronous Scans & Maps
- Steady State Fluorescence Anisotropy
- Excitation-Emission Maps (EEM)
- Kinetic Measurements
- Temperature Maps
Products
Applications
- Absolute Quantum Yield Measurements
- Steady State Singlet Oxygen Emission
- Water Quality Assessment
- Phosphor Powders Composition Analysis
- Fluorescence Upconversion
Publications
| Author | Year | Title | Journal | Additional details | Volume | Pages | Instruments | Publication details |
|---|---|---|---|---|---|---|---|---|
| Anna Gakamsky et al. | 2017 | Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level | Scientific Reports Volume:7 Pages:40375 Year:2017 | Journal:Scientific Reports Volume:7 Pages:40375 Year:2017 Instruments | 7 | 40375 | Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level Author:Anna Gakamsky et al.Further info Journal:Scientific Reports Volume:7 Pages:40375 Year:2017 Instruments | |
| Cédric Mongin et al. | 2016 | Direct observation of triplet energy transfer from semiconductor nanocrystals | Science Volume:351 Pages:369-372 Year:2016 | Journal:Science Volume:351 Pages:369-372 Year:2016 Instruments | 351 | 369-372 | Direct observation of triplet energy transfer from semiconductor nanocrystals Author:Cédric Mongin et al.Further info Journal:Science Volume:351 Pages:369-372 Year:2016 Instruments | |
| Eli Zysman-Colman et al. | 2016 | Solution-Processable Silicon Phthalocyanines in Electroluminescent and Photovoltaic Devices | ACS Applied Materials & Interfaces Volume:8 Pages:9247-9253 Year:2016 | Journal:ACS Applied Materials & Interfaces Volume:8 Pages:9247-9253 Year:2016 Instruments | 8 | 9247-9253 | Solution-Processable Silicon Phthalocyanines in Electroluminescent and Photovoltaic Devices Author:Eli Zysman-Colman et al.Further info Journal:ACS Applied Materials & Interfaces Volume:8 Pages:9247-9253 Year:2016 Instruments | |
| M. A. Shipman, K. J. Ramhita and B. A. Blight | 2016 | Sensing a Bacillis anthracis biomarker with well-known OLED emitter EuTta3Phen | Journal of Materials Chemistry B Volume: Pages: Year:2016 | Journal:Journal of Materials Chemistry B Volume: Pages: Year:2016 Instruments | Sensing a Bacillis anthracis biomarker with well-known OLED emitter EuTta3Phen Author: M. A. Shipman, K. J. Ramhita and B. A. BlightFurther info | |||
| Xixi Qin et al. | 2016 | Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window | Nature Scientific Reports Volume:6 Pages:20275 Year:2016 | Journal:Nature Scientific Reports Volume:6 Pages:20275 Year:2016 Instruments | 6 | 20275 | Hybrid coordination-network-engineering for bridging cascaded channels to activate long persistent phosphorescence in the second biological window Author:Xixi Qin et al.Further info | |
| De-Chao Yu et al. | 2015 | Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells | Light: Science & Applications Volume:4 Pages:e344 Year:2015 | Journal:Light: Science & Applications Volume:4 Pages:e344 Year:2015 Instruments | 4 | e344 | Multi-photon quantum cutting in Gd2O2S:Tm3+ to enhance the photo-response of solar cells Author:De-Chao Yu et al.Further info | |
| Like Huanga et al. | 2016 | Efficient planar perovskite solar cells without a high temperature processed titanium dioxide electron transport layer | Solar Energy Materials and Solar Cells Volume:149 Pages:1-8 Year:2016 | Journal:Solar Energy Materials and Solar Cells Volume:149 Pages:1-8 Year:2016 Instruments | 149 | 1-8 | Efficient planar perovskite solar cells without a high temperature processed titanium dioxide electron transport layer Author:Like Huanga et al.Further info Journal:Solar Energy Materials and Solar Cells Volume:149 Pages:1-8 Year:2016 Instruments | |
| Yanyan Li et al. | 2014 | A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer | Inorganic Chemistry Volume:53 Pages:7668-7675 Year:2014 | Journal:Inorganic Chemistry Volume:53 Pages:7668-7675 Year:2014 Instruments | 53 | 7668-7675 | A Single-Component White-Emitting CaSr2Al2O6:Ce3+, Li+, Mn2+ Phosphor via Energy Transfer Author:Yanyan Li et al.Further info Journal:Inorganic Chemistry Volume:53 Pages:7668-7675 Year:2014 Instruments | |
| Chen Liao et. al | 2015 | Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplers | Nanoscale Volume: Pages: Year:2015 | Journal:Nanoscale Volume: Pages: Year:2015 Instruments | Bright white-light emission from Ag/SiO2/CdS-ZnS core/shell/shell plasmon couplers Author: Chen Liao et. alFurther info | |||
| Ahmad S. Sediq et al. | 2016 | No Touching! Abrasion of Adsorbed Protein Is the Root Cause of Subvisible Particle Formation During Stirring | Journal of Pharmaceutical Sciences Volume: Pages: Year:2016 | Journal:Journal of Pharmaceutical Sciences Volume: Pages: Year:2016 Instruments | No Touching! Abrasion of Adsorbed Protein Is the Root Cause of Subvisible Particle Formation During Stirring Author:Ahmad S. Sediq et al.Further info | |||
| Yuren Wang, Haiching Ma | 2015 | Protein kinase profiling assays: a technology review | Drug Discovery Today: Technologies Volume:18 Pages:1-8 Year:2015 | Journal:Drug Discovery Today: Technologies Volume:18 Pages:1-8 Year:2015 Instruments | 18 | 1-8 | Protein kinase profiling assays: a technology review Author:Yuren Wang, Haiching MaFurther info | |
| Christos Pliotas et al. | 2015 | The role of lipids in mechanosensation | Nature Structural & Molecular Biology Volume:22 Pages:991-998 Year:2015 | Journal:Nature Structural & Molecular Biology Volume:22 Pages:991-998 Year:2015 Instruments | 22 | 991-998 | The role of lipids in mechanosensation Author:Christos Pliotas et al.Further info Journal:Nature Structural & Molecular Biology Volume:22 Pages:991-998 Year:2015 Instruments | |
| Choi, M. K. et al. | 2015 | Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing | Nature Communications Volume:6 Pages:7149 Year:2015 | Journal:Nature Communications Volume:6 Pages:7149 Year:2015 Instruments | 6 | 7149 | Wearable red-green-blue quantum dot light-emitting diode array using high-resolution intaglio transfer printing Author:Choi, M. K. et al.Further info Journal:Nature Communications Volume:6 Pages:7149 Year:2015 Instruments | |
| Bilel Louahem M’Sabah et al. | 2015 | Amplification of light collection in solid-state dye-sensitized solar cells via the antenna effect through supramolecular assembly | Physical Chemistry Chemical Physics Volume:17 Pages:9910-9918 Year:2015 | Journal:Physical Chemistry Chemical Physics Volume:17 Pages:9910-9918 Year:2015 Instruments | 17 | 9910-9918 | Amplification of light collection in solid-state dye-sensitized solar cells via the antenna effect through supramolecular assembly Author:Bilel Louahem M’Sabah et al.Further info Journal:Physical Chemistry Chemical Physics Volume:17 Pages:9910-9918 Year:2015 Instruments | |
| Ruichan Lva et al. | 2015 | An imaging-guided platform for synergistic photodynamic/photothermal/chemo-therapy with pH/temperature-responsive drug release | Biomaterials Volume:63 Pages:115-127 Year:2015 | Journal:Biomaterials Volume:63 Pages:115-127 Year:2015 Instruments | 63 | 115-127 | An imaging-guided platform for synergistic photodynamic/photothermal/chemo-therapy with pH/temperature-responsive drug release Author:Ruichan Lva et al.Further info | |
| Thomas J. Macdonald et al. | 2014 | Cation exchange of aqueous CuInS2 quantum dots | CrystEngComm Volume:16 Pages:9455-9460 Year:2014 | Journal:CrystEngComm Volume:16 Pages:9455-9460 Year:2014 Instruments | 16 | 9455-9460 | Cation exchange of aqueous CuInS2 quantum dots Author:Thomas J. Macdonald et al.Further info | |
| Renato Grillo et al. | 2015 | Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity | Journal of Hazardous Materials Volume:286 Pages:562-572 Year:2015 | Journal:Journal of Hazardous Materials Volume:286 Pages:562-572 Year:2015 Instruments | 286 | 562-572 | Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity Author:Renato Grillo et al.Further info Journal:Journal of Hazardous Materials Volume:286 Pages:562-572 Year:2015 Instruments |